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+--------+-------------------------+-------------------------+--------+--------+
|00000000| 00 13 4d 61 74 65 72 69 | 61 6c 73 20 26 20 4d 65 |..Materi|als & Me|
|00000010| 74 68 6f 64 73 00 00 00 | 00 00 00 00 00 00 00 00 |thods...|........|
|00000020| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000030| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000040| 00 53 54 41 4b 57 49 4c | 44 01 00 00 00 00 00 00 |.STAKWIL|D.......|
|00000050| 00 00 00 00 03 99 a0 00 | 00 1b df a3 4a 4c 46 a9 |........|....JLF.|
|00000060| e4 14 17 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000070| 00 00 00 00 00 00 00 00 | 00 00 81 81 e6 4e 00 00 |........|.....N..|
|00000080| 00 00 20 00 53 54 41 4b | ff ff ff ff 00 00 00 00 |.. .STAK|........|
|00000090| 00 00 00 0a 00 03 99 a0 | 00 00 20 00 00 00 00 00 |........|.. .....|
|000000a0| 00 00 00 00 00 00 00 01 | 00 00 0a 18 00 00 00 39 |........|.......9|
|000000b0| 00 00 0b cf 00 00 4c 06 | 00 00 00 00 00 00 00 00 |......L.|........|
|000000c0| 00 00 16 d7 00 00 00 00 | 00 05 00 00 10 00 00 00 |........|........|
|000000d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000000e0| 02 00 80 00 02 00 80 00 | 02 00 80 00 02 10 80 01 |........|........|
|000000f0| dd ae e9 e3 00 00 00 00 | 00 35 00 10 01 8b 02 10 |........|.5......|
|00000100| 00 00 00 00 02 70 03 40 | 00 00 00 00 00 00 00 00 |.....p.@|........|
|00000110| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000120| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000130| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000140| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000150| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000160| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000170| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000180| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000190| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000200| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000210| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000220| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000230| 00 00 0d 5d 00 00 0c a7 | 01 56 02 00 00 00 00 00 |...]....|.V......|
|00000240| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000250| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000260| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000270| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000280| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000290| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000300| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000310| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000320| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000330| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000340| 00 00 00 00 00 00 00 00 | 80 00 00 00 08 00 00 00 |........|........|
|00000350| 88 00 22 00 88 00 22 00 | 88 88 22 22 88 88 22 22 |.."...".|..""..""|
|00000360| 88 aa 22 aa 88 aa 22 aa | cc aa 33 aa cc aa 33 aa |.."...".|..3...3.|
|00000370| ee aa bb aa ee aa bb aa | ee bb bb ee ee bb bb ee |........|........|
|00000380| ff bb ff ee ff bb ff ee | ff bb ff ff ff bb ff ff |........|........|
|00000390| 80 10 02 20 01 08 40 04 | ff ff ff ff ff ff ff ff |... ..@.|........|
|000003a0| 88 22 88 22 88 22 88 22 | 11 22 44 88 11 22 44 88 |."."."."|."D.."D.|
|000003b0| c4 80 0c 68 43 02 30 26 | b1 30 03 1b d8 c0 0c 8d |...hC.0&|.0......|
|000003c0| aa 00 aa 00 aa 00 aa 00 | 88 22 55 22 88 22 55 22 |........|."U"."U"|
|000003d0| 88 55 22 55 88 55 22 55 | 77 dd 77 dd 77 dd 77 dd |.U"U.U"U|w.w.w.w.|
|000003e0| 80 00 00 00 00 00 00 00 | aa 55 aa 55 aa 55 aa 55 |........|.U.U.U.U|
|000003f0| 03 84 48 30 0c 02 01 01 | 82 44 39 44 82 01 01 01 |..H0....|.D9D....|
|00000400| 88 14 22 41 88 41 22 14 | 80 80 41 3e 08 08 14 e3 |.."A.A".|..A>....|
|00000410| 22 04 8c 74 22 17 98 10 | be 80 88 08 eb 08 88 80 |"..t"...|........|
|00000420| 25 c8 32 89 64 24 4c 92 | a2 9c 41 be 2a c9 14 eb |%.2.d$L.|..A.*...|
|00000430| 40 a0 00 00 04 0a 00 00 | 80 40 20 00 02 04 08 00 |@.......|.@ .....|
|00000440| aa 00 80 00 88 00 80 00 | ff 80 80 80 80 80 80 80 |........|........|
|00000450| 08 1c 22 c1 80 01 02 04 | ff 80 80 80 ff 08 08 08 |..".....|........|
|00000460| f8 74 22 47 8f 17 22 71 | bf 00 bf bf b0 b0 b0 b0 |.t"G.."q|........|
|00000470| ff 7f be 5d a2 41 80 00 | fa f5 fa f5 a0 50 a0 50 |...].A..|.....P.P|
|00000480| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000490| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000500| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000510| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000520| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000530| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000540| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000550| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000560| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000570| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000580| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000590| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000600| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000610| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000620| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000630| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000640| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000650| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000660| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000670| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000680| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000690| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000006a0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000006b0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000006c0| 2d 2d 2d 2d 2d 0d 2d 2d | 0d 2d 2d 20 4d 61 74 65 |-----.--|.-- Mate|
|000006d0| 72 69 61 6c 73 20 26 20 | 4d 65 74 68 6f 64 73 20 |rials & |Methods |
|000006e0| 76 65 72 73 69 6f 6e 20 | 35 2e 30 0d 2d 2d 0d 2d |version |5.0.--.-|
|000006f0| 2d 20 61 64 61 70 74 65 | 64 20 66 72 6f 6d 20 4d |- adapte|d from M|
|00000700| 26 4d 20 76 34 2e 32 20 | 31 30 2f 39 30 0d 2d 2d |&M v4.2 |10/90.--|
|00000710| 20 66 6f 72 20 48 79 70 | 65 72 63 61 72 64 20 76 | for Hyp|ercard v|
|00000720| 32 2e 30 0d 2d 2d 0d 2d | 2d 20 62 79 20 4a 61 6d |2.0.--.-|- by Jam|
|00000730| 65 73 20 57 2e 20 42 72 | 6f 77 6e 0d 2d 2d 20 42 |es W. Br|own.-- B|
|00000740| 69 6f 6c 6f 67 79 20 44 | 65 70 61 72 74 6d 65 6e |iology D|epartmen|
|00000750| 74 0d 2d 2d 20 49 6e 64 | 69 61 6e 61 20 55 6e 69 |t.-- Ind|iana Uni|
|00000760| 76 65 72 73 69 74 79 0d | 2d 2d 20 42 6c 6f 6f 6d |versity.|-- Bloom|
|00000770| 69 6e 67 74 6f 6e 2c 20 | 49 4e 20 34 37 34 30 35 |ington, |IN 47405|
|00000780| 0d 2d 2d 20 55 53 41 0d | 2d 2d 0d 2d 2d 20 42 69 |.-- USA.|--.-- Bi|
|00000790| 74 4e 65 74 20 61 64 64 | 72 65 73 73 20 3a 20 42 |tNet add|ress : B|
|000007a0| 52 4f 57 4e 4a 57 40 49 | 55 42 41 43 53 0d 2d 2d |ROWNJW@I|UBACS.--|
|000007b0| 0d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |.-------|--------|
|000007c0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000007d0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000007e0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000007f0| 2d 2d 2d 2d 2d 2d 0d 2d | 2d 20 47 6c 6f 62 61 6c |------.-|- Global|
|00000800| 73 3a 20 54 48 45 4c 49 | 53 54 20 2d 20 61 20 6c |s: THELI|ST - a l|
|00000810| 69 73 74 20 6f 66 20 63 | 61 72 64 20 6e 61 6d 65 |ist of c|ard name|
|00000820| 73 20 66 6f 72 20 74 68 | 65 20 22 43 68 6f 6f 73 |s for th|e "Choos|
|00000830| 65 20 63 61 72 64 22 20 | 66 75 6e 63 74 69 6f 6e |e card" |function|
|00000840| 0d 2d 2d 20 20 20 20 20 | 20 20 20 20 54 48 45 54 |.--     |    THET|
|00000850| 41 4c 4c 59 20 2d 20 75 | 73 65 64 20 6f 6e 6c 79 |ALLY - u|sed only|
|00000860| 20 62 79 20 22 6f 6e 20 | 69 64 6c 65 22 20 74 6f | by "on |idle" to|
|00000870| 20 70 75 74 20 61 77 61 | 79 20 74 68 65 20 6d 73 | put awa|y the ms|
|00000880| 67 20 62 6f 78 0d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |g box.--|--------|
|00000890| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000008a0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000008b0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000008c0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 0d 2d 2d 20 58 |--------|---.-- X|
|000008d0| 43 4d 44 53 20 75 73 65 | 64 20 3a 20 64 6f 4c 69 |CMDS use|d : doLi|
|000008e0| 73 74 20 2d 20 63 72 65 | 61 74 65 73 20 74 68 65 |st - cre|ates the|
|000008f0| 20 73 63 72 6f 6c 6c 69 | 6e 67 20 6c 69 73 74 20 | scrolli|ng list |
|00000900| 66 6f 72 20 22 43 68 6f | 6f 73 65 20 63 61 72 64 |for "Cho|ose card|
|00000910| 22 0d 2d 2d 20 20 20 20 | 20 64 6f 4c 69 73 74 20 |".--    | doList |
|00000920| 69 73 20 69 6e 73 74 61 | 6c 6c 65 64 20 69 6e 20 |is insta|lled in |
|00000930| 74 68 65 20 73 74 61 63 | 6b 0d 2d 2d 2d 2d 2d 2d |the stac|k.------|
|00000940| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000950| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000960| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000970| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 0d |--------|-------.|
|00000980| 2d 2d 20 4e 6f 6e 2d 73 | 74 61 6e 64 61 72 64 20 |-- Non-s|tandard |
|00000990| 69 63 6f 6e 73 20 75 73 | 65 64 20 3a 20 74 72 6f |icons us|ed : tro|
|000009a0| 70 69 63 61 6c 20 68 6f | 6d 65 2c 20 64 69 73 6b |pical ho|me, disk|
|000009b0| 0d 2d 2d 20 20 20 20 20 | 62 6f 74 68 20 61 72 65 |.--     |both are|
|000009c0| 20 69 6e 73 74 61 6c 6c | 65 64 20 69 6e 20 74 68 | install|ed in th|
|000009d0| 65 20 73 74 61 63 6b 0d | 2d 2d 2d 2d 2d 2d 2d 2d |e stack.|--------|
|000009e0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000009f0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000a00| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000a10| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 0d 2d 2d |--------|-----.--|
|00000a20| 20 54 68 69 73 20 73 74 | 61 63 6b 20 61 6c 73 6f | This st|ack also|
|00000a30| 20 63 6f 6e 74 61 69 6e | 73 20 50 49 43 54 20 61 | contain|s PICT a|
|00000a40| 6e 64 20 50 4c 54 45 20 | 72 65 73 6f 75 72 63 65 |nd PLTE |resource|
|00000a50| 73 20 69 6e 73 74 61 6c | 6c 65 64 20 62 79 20 74 |s instal|led by t|
|00000a60| 68 65 0d 2d 2d 20 70 61 | 6c 65 74 74 65 20 6d 61 |he.-- pa|lette ma|
|00000a70| 6b 65 72 20 66 6f 72 20 | 75 73 65 20 62 79 20 74 |ker for |use by t|
|00000a80| 68 65 20 22 41 62 6f 75 | 74 2e 2e 2e 22 20 62 75 |he "Abou|t..." bu|
|00000a90| 74 74 6f 6e 20 6d 6f 75 | 73 65 55 70 20 68 61 6e |tton mou|seUp han|
|00000aa0| 64 6c 65 72 0d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |dler.---|--------|
|00000ab0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000ac0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000ad0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000ae0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 0d 0d 0d 0d 2d 2d |--------|--....--|
|00000af0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000b00| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000b10| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000b20| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000b30| 2d 2d 2d 0d 2d 2d 20 48 | 6f 75 73 65 6b 65 65 70 |---.-- H|ousekeep|
|00000b40| 69 6e 67 20 73 63 72 69 | 70 74 73 0d 2d 2d 0d 0d |ing scri|pts.--..|
|00000b50| 6f 6e 20 6f 70 65 6e 53 | 74 61 63 6b 0d 67 6c 6f |on openS|tack.glo|
|00000b60| 62 61 6c 20 54 48 45 4c | 49 53 54 0d 70 75 74 20 |bal THEL|IST.put |
|00000b70| 22 4f 6e 65 20 6d 6f 6d | 65 6e 74 2c 20 70 6c 65 |"One mom|ent, ple|
|00000b80| 61 73 65 2e 2e 2e 2e 22 | 20 69 6e 74 6f 20 6d 73 |ase...."| into ms|
|00000b90| 67 0d 73 65 74 20 74 68 | 65 20 63 75 72 73 6f 72 |g.set th|e cursor|
|00000ba0| 20 74 6f 20 77 61 74 63 | 68 0d 2d 2d 4c 6f 61 64 | to watc|h.--Load|
|00000bb0| 20 75 70 20 54 48 45 4c | 49 53 54 0d 69 66 20 74 | up THEL|IST.if t|
|00000bc0| 68 65 20 6e 61 6d 65 20 | 6f 66 20 74 68 69 73 20 |he name |of this |
|00000bd0| 63 61 72 64 20 69 73 20 | 6e 6f 74 20 22 4d 26 4d |card is |not "M&M|
|00000be0| 20 69 6e 74 72 6f 64 75 | 63 74 69 6f 6e 22 20 74 | introdu|ction" t|
|00000bf0| 68 65 6e 0d 70 75 73 68 | 20 63 61 72 64 0d 67 6f |hen.push| card.go|
|00000c00| 20 74 6f 20 63 61 72 64 | 20 22 4d 26 4d 20 69 6e | to card| "M&M in|
|00000c10| 74 72 6f 64 75 63 74 69 | 6f 6e 22 0d 70 75 74 20 |troducti|on".put |
|00000c20| 63 61 72 64 20 66 69 65 | 6c 64 20 22 6c 69 73 74 |card fie|ld "list|
|00000c30| 46 69 65 6c 64 22 20 69 | 6e 74 6f 20 54 48 45 4c |Field" i|nto THEL|
|00000c40| 49 53 54 0d 70 6f 70 20 | 63 61 72 64 0d 65 6c 73 |IST.pop |card.els|
|00000c50| 65 0d 70 75 74 20 63 61 | 72 64 20 66 69 65 6c 64 |e.put ca|rd field|
|00000c60| 20 22 6c 69 73 74 46 69 | 65 6c 64 22 20 69 6e 74 | "listFi|eld" int|
|00000c70| 6f 20 54 48 45 4c 49 53 | 54 0d 65 6e 64 20 69 66 |o THELIS|T.end if|
|00000c80| 0d 2d 2d 20 49 73 20 54 | 48 45 4c 49 53 54 20 4f |.-- Is T|HELIST O|
|00000c90| 4b 3f 0d 69 66 20 28 74 | 68 65 20 6e 75 6d 62 65 |K?.if (t|he numbe|
|00000ca0| 72 20 6f 66 20 63 61 72 | 64 73 20 69 6e 20 74 68 |r of car|ds in th|
|00000cb0| 69 73 20 73 74 61 63 6b | 29 20 ad 20 28 74 68 65 |is stack|) . (the|
|00000cc0| 20 6e 75 6d 62 65 72 20 | 6f 66 20 69 74 65 6d 73 | number |of items|
|00000cd0| 20 69 6e 20 54 48 45 4c | 49 53 54 29 20 74 68 65 | in THEL|IST) the|
|00000ce0| 6e 0d 70 75 74 20 22 53 | 6f 72 72 79 20 2d 20 49 |n.put "S|orry - I|
|00000cf0| 20 68 61 76 65 20 74 6f | 20 72 65 2d 69 6e 64 65 | have to| re-inde|
|00000d00| 78 20 74 68 65 20 63 61 | 72 64 73 2e 2e 2e 22 20 |x the ca|rds..." |
|00000d10| 69 6e 74 6f 20 6d 73 67 | 0d 63 72 65 61 74 65 4c |into msg|.createL|
|00000d20| 69 73 74 0d 65 6e 64 20 | 69 66 0d 70 75 74 20 65 |ist.end |if.put e|
|00000d30| 6d 70 74 79 20 69 6e 74 | 6f 20 6d 73 67 0d 68 69 |mpty int|o msg.hi|
|00000d40| 64 65 20 6d 73 67 0d 65 | 6e 64 20 6f 70 65 6e 73 |de msg.e|nd opens|
|00000d50| 74 61 63 6b 0d 0d 6f 6e | 20 63 6c 6f 73 65 53 74 |tack..on| closeSt|
|00000d60| 61 63 6b 0d 67 6c 6f 62 | 61 6c 20 54 48 45 4c 49 |ack.glob|al THELI|
|00000d70| 53 54 0d 2d 2d 20 6e 75 | 6b 65 20 74 68 65 20 22 |ST.-- nu|ke the "|
|00000d80| 41 62 6f 75 74 2e 2e 2e | 22 20 70 61 6c 65 74 74 |About...|" palett|
|00000d90| 65 0d 63 6c 6f 73 65 20 | 77 69 6e 64 6f 77 20 22 |e.close |window "|
|00000da0| 61 62 6f 75 74 2e 2e 2e | 22 0d 2d 2d 20 75 70 64 |about...|".-- upd|
|00000db0| 61 74 65 20 6c 69 73 74 | 46 69 65 6c 64 0d 67 6f |ate list|Field.go|
|00000dc0| 20 74 6f 20 63 61 72 64 | 20 22 4d 26 4d 20 69 6e | to card| "M&M in|
|00000dd0| 74 72 6f 64 75 63 74 69 | 6f 6e 22 0d 70 75 74 20 |troducti|on".put |
|00000de0| 54 48 45 4c 49 53 54 20 | 69 6e 74 6f 20 63 61 72 |THELIST |into car|
|00000df0| 64 20 66 69 65 6c 64 20 | 22 6c 69 73 74 46 69 65 |d field |"listFie|
|00000e00| 6c 64 22 0d 70 75 74 20 | 65 6d 70 74 79 20 69 6e |ld".put |empty in|
|00000e10| 74 6f 20 54 48 45 4c 49 | 53 54 0d 65 6e 64 20 63 |to THELI|ST.end c|
|00000e20| 6c 6f 73 65 53 74 61 63 | 6b 0d 0d 6f 6e 20 69 64 |loseStac|k..on id|
|00000e30| 6c 65 0d 2d 2d 20 49 66 | 20 74 68 65 20 6d 73 67 |le.-- If| the msg|
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|00004930| 4e 45 52 41 4c 20 49 4e | 46 4f 52 4d 41 54 49 4f |NERAL IN|FORMATIO|
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|00004950| 20 26 20 4d 65 74 68 6f | 64 73 20 28 4d 26 4d 29 | & Metho|ds (M&M)|
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|00004a50| 73 65 72 69 65 73 20 28 | 6c 6f 74 73 21 29 20 6f |series (|lots!) o|
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|00004ac0| 79 20 61 64 64 65 64 2e | 0d 0d 53 59 53 54 45 4d |y added.|..SYSTEM|
|00004ad0| 20 52 45 51 55 49 52 45 | 4d 45 4e 54 53 0d 20 20 | REQUIRE|MENTS.  |
|00004ae0| 20 20 20 54 68 69 73 20 | 76 65 72 73 69 6f 6e 20 |   This |version |
|00004af0| 6f 66 20 4d 26 4d 20 28 | 35 2e 30 29 20 72 65 71 |of M&M (|5.0) req|
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|00004b10| 76 65 72 73 69 6f 6e 20 | 32 2e 30 2c 20 6f 72 20 |version |2.0, or |
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|00004b40| 30 20 69 73 6e 27 74 20 | 65 76 65 6e 20 72 65 61 |0 isn't |even rea|
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|00004b60| 20 61 73 20 49 20 77 72 | 69 74 65 20 74 68 69 73 | as I wr|ite this|
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|00004b90| 65 71 75 69 72 65 73 20 | 53 79 73 74 65 6d 20 36 |equires |System 6|
|00004ba0| 2e 30 2e 35 20 28 6f 72 | 20 67 72 65 61 74 65 72 |.0.5 (or| greater|
|00004bb0| 29 2e 20 20 41 6e 79 20 | 4d 61 63 69 6e 74 6f 73 |).  Any |Macintos|
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|00004bd0| 6c 65 20 6f 66 20 72 75 | 6e 6e 69 6e 67 20 48 79 |le of ru|nning Hy|
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|00004c10| 20 31 4d 62 79 74 65 20 | 6f 66 20 52 41 4d 2c 20 | 1Mbyte |of RAM, |
|00004c20| 61 6e 64 20 68 6f 70 65 | 66 75 6c 6c 79 20 61 20 |and hope|fully a |
|00004c30| 68 61 72 64 20 64 69 73 | 6b 2e 20 20 4d 26 4d 20 |hard dis|k.  M&M |
|00004c40| 63 61 72 64 73 20 61 72 | 65 20 74 68 65 20 73 74 |cards ar|e the st|
|00004c50| 61 6e 64 61 72 64 20 39 | 22 20 73 69 7a 65 2c 20 |andard 9|" size, |
|00004c60| 73 6f 20 74 68 61 74 20 | 74 68 65 20 65 6e 74 69 |so that |the enti|
|00004c70| 72 65 20 63 61 72 64 20 | 73 68 6f 75 6c 64 20 62 |re card |should b|
|00004c80| 65 20 76 69 73 69 62 6c | 65 20 6f 6e 20 4d 61 63 |e visibl|e on Mac|
|00004c90| 2b 2c 20 53 45 2c 20 6f | 72 20 22 43 6c 61 73 73 |+, SE, o|r "Class|
|00004ca0| 69 63 22 20 73 63 72 65 | 65 6e 73 2e 20 20 59 6f |ic" scre|ens.  Yo|
|00004cb0| 75 20 73 68 6f 75 6c 64 | 20 62 65 20 66 61 6d 69 |u should| be fami|
|00004cc0| 6c 69 61 72 20 77 69 74 | 68 20 74 68 65 20 4d 61 |liar wit|h the Ma|
|00004cd0| 63 69 6e 74 6f 73 68 20 | 65 6e 76 69 72 6f 6e 6d |cintosh |environm|
|00004ce0| 65 6e 74 20 69 6e 20 67 | 65 6e 65 72 61 6c 2c 20 |ent in g|eneral, |
|00004cf0| 61 6e 64 20 74 68 65 20 | 48 79 70 65 72 63 61 72 |and the |Hypercar|
|00004d00| 64 20 65 6e 76 69 72 6f | 6e 6d 65 6e 74 20 69 6e |d enviro|nment in|
|00004d10| 20 73 70 65 63 69 66 69 | 63 2c 20 62 65 66 6f 72 | specifi|c, befor|
|00004d20| 65 20 75 73 69 6e 67 20 | 4d 26 4d 2e 0d 0d 49 4e |e using |M&M...IN|
|00004d30| 54 52 4f 44 55 43 54 49 | 4f 4e 0d 20 20 20 20 20 |TRODUCTI|ON.     |
|00004d40| 4d 26 4d 20 73 74 6f 72 | 65 73 20 65 61 63 68 20 |M&M stor|es each |
|00004d50| 70 72 6f 74 6f 63 6f 6c | 20 6f 6e 20 6f 6e 65 20 |protocol| on one |
|00004d60| 63 61 72 64 2c 20 69 6e | 20 61 20 73 63 72 6f 6c |card, in| a scrol|
|00004d70| 6c 69 6e 67 20 77 69 6e | 64 6f 77 20 6a 75 73 74 |ling win|dow just|
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|00004d90| 20 57 68 65 6e 20 4d 26 | 4d 20 69 73 20 73 74 61 | When M&|M is sta|
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|00004db0| 6f 64 75 63 74 69 6f 6e | 22 20 63 61 72 64 20 69 |oduction|" card i|
|00004dc0| 73 20 73 68 6f 77 6e 2e | 20 20 54 6f 20 62 72 6f |s shown.|  To bro|
|00004dd0| 77 73 65 20 74 68 72 6f | 75 67 68 20 74 68 65 20 |wse thro|ugh the |
|00004de0| 63 61 72 64 73 2c 20 65 | 69 74 68 65 72 20 75 73 |cards, e|ither us|
|00004df0| 65 20 74 68 65 20 61 72 | 72 6f 77 20 62 75 74 74 |e the ar|row butt|
|00004e00| 6f 6e 73 20 74 6f 20 74 | 68 65 20 72 69 67 68 74 |ons to t|he right|
|00004e10| 20 6f 66 20 74 68 65 20 | 63 61 72 64 2c 20 6f 72 | of the |card, or|
|00004e20| 20 63 6c 69 63 6b 20 74 | 68 65 20 22 43 68 6f 6f | click t|he "Choo|
|00004e30| 73 65 20 63 61 72 64 22 | 20 62 75 74 74 6f 6e 2e |se card"| button.|
|00004e40| 20 20 22 43 68 6f 6f 73 | 65 20 63 61 72 64 22 20 |  "Choos|e card" |
|00004e50| 62 72 69 6e 67 73 20 75 | 70 20 28 61 66 74 65 72 |brings u|p (after|
|00004e60| 20 61 6e 20 69 6e 74 65 | 72 6d 69 6e 61 62 6c 65 | an inte|rminable|
|00004e70| 20 64 65 6c 61 79 29 20 | 61 20 73 63 72 6f 6c 6c | delay) |a scroll|
|00004e80| 69 6e 67 20 6c 69 73 74 | 20 6f 66 20 61 6c 6c 20 |ing list| of all |
|00004e90| 6f 66 20 74 68 65 20 70 | 72 6f 74 6f 63 6f 6c 20 |of the p|rotocol |
|00004ea0| 63 61 72 64 73 20 69 6e | 20 74 68 65 20 73 74 61 |cards in| the sta|
|00004eb0| 63 6b 20 2d 20 6a 75 73 | 74 20 64 6f 75 62 6c 65 |ck - jus|t double|
|00004ec0| 20 63 6c 69 63 6b 20 6f | 6e 20 74 68 65 20 6f 6e | click o|n the on|
|00004ed0| 65 20 79 6f 75 20 77 61 | 6e 74 20 74 6f 20 67 6f |e you wa|nt to go|
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|000053d0| 20 50 52 4f 54 4f 43 4f | 4c 20 43 41 52 44 53 2e | PROTOCO|L CARDS.|
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|00005590| 20 20 49 66 20 74 68 65 | 20 63 61 72 64 20 61 6c |  If the| card al|
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|000055b0| 20 70 72 6f 74 6f 63 6f | 6c 2c 20 61 20 6e 65 77 | protoco|l, a new|
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|00005640| 48 41 4e 47 45 53 20 49 | 4e 20 56 45 52 53 49 4f |HANGES I|N VERSIO|
|00005650| 4e 20 35 2e 30 0d 20 20 | 20 20 20 4d 26 4d 20 35 |N 5.0.  |   M&M 5|
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|00005670| 63 6f 6e 74 69 6e 75 61 | 74 69 6f 6e 20 6f 66 20 |continua|tion of |
|00005680| 74 68 65 20 74 72 65 6e | 64 20 74 6f 77 61 72 64 |the tren|d toward|
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|000056b0| 62 79 20 70 72 65 76 69 | 6f 75 73 20 76 65 72 73 |by previ|ous vers|
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|000056d0| 20 70 6f 69 6e 74 65 64 | 20 6f 75 74 20 74 6f 20 | pointed| out to |
|000056e0| 6d 65 20 74 68 61 74 2c | 20 6c 69 6b 65 20 6d 61 |me that,| like ma|
|000056f0| 6e 79 20 63 6f 6d 70 75 | 74 65 72 20 70 72 6f 67 |ny compu|ter prog|
|00005700| 72 61 6d 73 2c 20 4d 26 | 4d 20 69 74 20 77 69 6c |rams, M&|M it wil|
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|00005720| 20 74 68 65 20 70 65 61 | 6b 20 6f 66 20 73 6f 66 | the pea|k of sof|
|00005730| 74 77 61 72 65 20 70 65 | 72 66 65 63 74 69 6f 6e |tware pe|rfection|
|00005740| 20 61 74 20 65 78 61 63 | 74 6c 79 20 74 68 61 74 | at exac|tly that|
|00005750| 20 70 6f 69 6e 74 20 69 | 6e 20 77 68 69 63 68 20 | point i|n which |
|00005760| 69 74 20 63 65 61 73 65 | 73 20 65 6e 74 69 72 65 |it cease|s entire|
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|00005780| 20 76 65 72 73 69 6f 6e | 20 35 2e 30 2c 20 74 68 | version| 5.0, th|
|00005790| 65 20 22 4d 26 4d 20 55 | 74 69 6c 69 74 69 65 73 |e "M&M U|tilities|
|000057a0| 22 20 73 74 61 63 6b 20 | 74 68 61 74 20 63 61 6d |" stack |that cam|
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|000057c0| 76 65 72 73 69 6f 6e 73 | 20 69 73 20 67 6f 6e 65 |versions| is gone|
|000057d0| 20 2d 20 4d 26 4d 20 69 | 73 20 6e 6f 77 20 28 49 | - M&M i|s now (I|
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|00005830| 67 20 62 65 74 77 65 65 | 6e 20 77 69 6e 64 6f 77 |g betwee|n window|
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|00005870| 63 74 20 4c 61 73 65 72 | 57 72 69 74 65 72 20 70 |ct Laser|Writer p|
|00005880| 72 69 6e 74 69 6e 67 2e | 0d 0d 43 4f 4e 43 4c 55 |rinting.|..CONCLU|
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|000058b0| 65 20 69 73 20 74 6f 20 | 4d 26 4d 2e 20 20 49 66 |e is to |M&M.  If|
|000058c0| 20 79 6f 75 20 68 61 76 | 65 20 61 6e 79 20 63 6f | you hav|e any co|
|000058d0| 6d 6d 65 6e 74 73 2c 20 | 71 75 65 73 74 69 6f 6e |mments, |question|
|000058e0| 73 2c 20 70 72 6f 62 6c | 65 6d 73 2c 20 62 75 67 |s, probl|ems, bug|
|000058f0| 20 72 65 70 6f 72 74 73 | 2c 20 65 74 63 2c 20 70 | reports|, etc, p|
|00005900| 6c 65 61 73 65 20 66 65 | 65 6c 20 66 72 65 65 20 |lease fe|el free |
|00005910| 74 6f 20 63 6f 6e 74 61 | 63 74 20 6d 65 20 61 74 |to conta|ct me at|
|00005920| 3a 0d 0d 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |:..     |        |
|00005930| 20 20 20 20 20 20 20 20 | 20 4a 61 6d 65 73 20 57 |        | James W|
|00005940| 2e 20 42 72 6f 77 6e 0d | 20 20 20 20 20 20 20 20 |. Brown.|        |
|00005950| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 44 65 |        |      De|
|00005960| 70 61 72 74 6d 65 6e 74 | 20 6f 66 20 42 69 6f 6c |partment| of Biol|
|00005970| 6f 67 79 0d 20 20 20 20 | 20 20 20 20 20 20 20 20 |ogy.    |        |
|00005980| 20 20 20 20 20 20 20 20 | 20 20 49 6e 64 69 61 6e |        |  Indian|
|00005990| 61 20 75 6e 69 76 65 72 | 73 69 74 79 0d 20 20 20 |a univer|sity.   |
|000059a0| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
|000059b0| 20 20 20 42 6c 6f 6f 6d | 69 6e 67 74 6f 6e 2c 20 |   Bloom|ington, |
|000059c0| 49 4e 20 34 37 34 30 35 | 20 20 55 53 41 0d 0d 6f |IN 47405|  USA..o|
|000059d0| 72 20 62 79 20 42 69 74 | 6e 65 74 20 61 74 20 42 |r by Bit|net at B|
|000059e0| 52 4f 57 4e 4a 57 40 49 | 55 42 41 43 53 2e 20 20 |ROWNJW@I|UBACS.  |
|000059f0| 49 20 61 6d 20 61 6c 73 | 6f 20 68 61 70 70 79 20 |I am als|o happy |
|00005a00| 74 6f 20 67 69 76 65 20 | 6f 75 74 20 75 70 64 61 |to give |out upda|
|00005a10| 74 65 73 20 6f 66 20 61 | 6e 79 20 6f 66 20 6d 79 |tes of a|ny of my|
|00005a20| 20 73 74 61 63 6b 73 20 | 75 70 6f 6e 20 72 65 71 | stacks |upon req|
|00005a30| 75 65 73 74 2e 20 20 54 | 68 69 73 20 69 73 20 6d |uest.  T|his is m|
|00005a40| 6f 73 74 20 65 61 73 69 | 6c 79 20 28 66 6f 72 20 |ost easi|ly (for |
|00005a50| 6d 65 29 20 64 6f 6e 65 | 20 62 79 20 45 2d 6d 61 |me) done| by E-ma|
|00005a60| 69 6c 2c 20 62 75 74 20 | 63 61 6e 20 61 6c 73 6f |il, but |can also|
|00005a70| 20 62 65 20 61 63 63 6f | 6d 70 6c 69 73 68 65 64 | be acco|mplished|
|00005a80| 20 62 79 20 73 6c 6f 77 | 2d 6d 61 69 6c 2e 20 20 | by slow|-mail.  |
|00005a90| 4d 6f 73 74 20 6f 66 20 | 6d 79 20 73 74 75 66 66 |Most of |my stuff|
|00005aa0| 20 69 73 20 61 6c 73 6f | 20 61 76 61 69 6c 61 62 | is also| availab|
|00005ab0| 6c 65 20 66 72 6f 6d 20 | 74 68 65 20 45 4d 42 4c |le from |the EMBL|
|00005ac0| 20 66 69 6c 65 73 65 72 | 76 65 72 2e 20 20 46 65 | fileser|ver.  Fe|
|00005ad0| 65 6c 20 66 72 65 65 20 | 74 6f 20 63 6f 70 79 20 |el free |to copy |
|00005ae0| 6f 72 20 67 69 76 65 20 | 61 77 61 79 20 4d 26 4d |or give |away M&M|
|00005af0| 2c 20 6f 6e 20 74 68 65 | 20 6f 66 66 20 63 68 61 |, on the| off cha|
|00005b00| 6e 63 65 20 74 68 61 74 | 20 61 6e 79 6f 6e 65 20 |nce that| anyone |
|00005b10| 73 68 6f 75 6c 64 20 77 | 61 6e 74 20 69 74 2c 20 |should w|ant it, |
|00005b20| 62 75 74 20 61 62 73 6f | 6c 75 74 65 6c 79 20 70 |but abso|lutely p|
|00005b30| 6f 73 69 74 69 76 65 6c | 79 20 61 74 20 4e 4f 20 |ositivel|y at NO |
|00005b40| 43 48 41 52 47 45 20 57 | 48 41 54 45 56 45 52 2e |CHARGE W|HATEVER.|
|00005b50| 20 20 41 73 20 79 6f 75 | 20 6d 69 67 68 74 20 65 |  As you| might e|
|00005b60| 78 70 65 63 74 2c 20 49 | 20 63 61 6e 6e 6f 74 20 |xpect, I| cannot |
|00005b70| 67 75 61 72 61 6e 74 65 | 65 20 74 68 65 20 75 74 |guarante|e the ut|
|00005b80| 69 6c 69 74 79 20 6f 66 | 20 61 6e 79 20 70 72 6f |ility of| any pro|
|00005b90| 74 6f 63 6f 6c 20 74 68 | 61 74 20 6d 61 79 20 61 |tocol th|at may a|
|00005ba0| 70 70 65 61 72 20 77 69 | 74 68 69 6e 20 74 68 69 |ppear wi|thin thi|
|00005bb0| 73 20 73 74 61 63 6b 2e | 20 20 46 6f 6c 6c 6f 77 |s stack.|  Follow|
|00005bc0| 20 61 6e 79 20 70 72 6f | 63 65 64 75 72 65 20 77 | any pro|cedure w|
|00005bd0| 69 74 68 20 61 20 63 72 | 69 74 69 63 61 6c 20 65 |ith a cr|itical e|
|00005be0| 79 65 20 2d 20 74 68 65 | 20 70 65 72 73 6f 6e 20 |ye - the| person |
|00005bf0| 77 68 6f 20 74 79 70 65 | 64 20 69 6e 20 74 68 65 |who type|d in the|
|00005c00| 20 70 72 6f 74 6f 63 6f | 6c 20 77 61 73 20 70 72 | protoco|l was pr|
|00005c10| 6f 62 61 62 6c 79 20 69 | 6e 20 61 20 68 75 72 72 |obably i|n a hurr|
|00005c20| 79 21 20 20 49 66 20 79 | 6f 75 20 64 69 73 74 72 |y!  If y|ou distr|
|00005c30| 69 62 75 74 65 20 4d 26 | 4d 20 77 69 74 68 20 70 |ibute M&|M with p|
|00005c40| 72 6f 74 6f 63 6f 6c 73 | 20 79 6f 75 27 76 65 20 |rotocols| you've |
|00005c50| 61 64 64 65 64 2c 20 50 | 4c 45 41 53 45 20 62 65 |added, P|LEASE be|
|00005c60| 20 73 75 72 65 20 74 6f | 20 70 72 6f 6f 66 2d 72 | sure to| proof-r|
|00005c70| 65 61 64 20 74 68 65 6d | 21 20 20 47 6f 6f 64 20 |ead them|!  Good |
|00005c80| 6c 75 63 6b 21 0d 0d 0d | 20 20 20 20 20 20 20 20 |luck!...|        |
|00005c90| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
|00005ca0| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
|00005cb0| 20 20 20 20 20 4a 69 6d | 20 42 72 6f 77 6e 0d 00 |     Jim| Brown..|
|00005cc0| ff ff 03 ff 00 4d 26 4d | 20 49 6e 74 72 6f 64 75 |.....M&M| Introdu|
|00005cd0| 63 74 69 6f 6e 2c 43 6f | 6c 6f 6e 79 20 6c 69 66 |ction,Co|lony lif|
|00005ce0| 74 73 2c 43 73 43 6c 20 | 70 6c 61 73 6d 69 64 20 |ts,CsCl |plasmid |
|00005cf0| 70 72 65 70 73 2c 43 73 | 43 6c 20 52 4e 41 20 69 |preps,Cs|Cl RNA i|
|00005d00| 73 6f 6c 61 74 69 6f 6e | 2c 44 4d 53 2d 74 72 65 |solation|,DMS-tre|
|00005d10| 61 74 69 6e 67 20 44 4e | 41 2c 44 4e 61 73 65 20 |ating DN|A,DNase |
|00005d20| 49 20 66 6f 6f 74 70 72 | 69 6e 74 69 6e 67 2c 44 |I footpr|inting,D|
|00005d30| 53 20 63 44 4e 41 20 73 | 79 6e 74 68 65 73 69 73 |S cDNA s|ynthesis|
|00005d40| 2c 45 6e 64 2d 6c 61 62 | 65 6c 69 6e 67 20 44 4e |,End-lab|eling DN|
|00005d50| 41 2c 45 6e 64 2d 6c 61 | 62 65 6c 69 6e 67 20 52 |A,End-la|beling R|
|00005d60| 4e 41 2c 45 74 42 72 20 | 73 70 6f 74 20 74 65 73 |NA,EtBr |spot tes|
|00005d70| 74 2c 45 75 62 61 63 74 | 65 72 69 61 6c 20 52 4e |t,Eubact|erial RN|
|00005d80| 41 20 69 73 6f 6c 61 74 | 69 6f 6e 2c 45 78 6f 20 |A isolat|ion,Exo |
|00005d90| 49 49 49 20 66 6f 6f 74 | 70 72 69 6e 74 69 6e 67 |III foot|printing|
|00005da0| 2c 46 69 67 75 72 65 20 | 38 20 28 43 29 20 74 65 |,Figure |8 (C) te|
|00005db0| 73 74 2c 46 69 6c 74 65 | 72 2d 62 69 6e 64 69 6e |st,Filte|r-bindin|
|00005dc0| 67 20 61 73 73 61 79 2c | 47 69 61 6e 74 20 6d 69 |g assay,|Giant mi|
|00005dd0| 6e 69 2d 70 72 65 70 2c | 47 65 6e 6f 6d 69 63 20 |ni-prep,|Genomic |
|00005de0| 44 4e 41 20 69 73 6f 6c | 61 74 69 6f 6e 2c 47 6c |DNA isol|ation,Gl|
|00005df0| 79 6f 78 61 6c 20 67 65 | 6c 73 2c 48 79 62 72 69 |yoxal ge|ls,Hybri|
|00005e00| 64 69 7a 61 74 69 6f 6e | 2c 48 79 64 72 6f 6c 79 |dization|,Hydroly|
|00005e10| 7a 69 6e 67 20 52 4e 41 | 2c 48 79 64 72 6f 6c 79 |zing RNA|,Hydroly|
|00005e20| 7a 69 6e 67 20 52 4e 41 | 20 23 32 2c 31 36 53 20 |zing RNA| #2,16S |
|00005e30| 72 52 4e 41 20 53 65 71 | 75 65 6e 63 69 6e 67 2c |rRNA Seq|uencing,|
|00005e40| 4d 31 33 20 63 6c 6f 6e | 69 6e 67 2c 4d 61 6e 69 |M13 clon|ing,Mani|
|00005e50| 61 74 69 73 2c 4d 65 74 | 68 61 6e 6f 67 65 6e 20 |atis,Met|hanogen |
|00005e60| 63 75 6c 74 75 72 65 73 | 2c 46 65 72 6d 65 6e 74 |cultures|,Ferment|
|00005e70| 69 6e 67 20 6d 65 74 68 | 61 6e 6f 67 65 6e 73 2c |ing meth|anogens,|
|00005e80| 4d 69 63 72 6f 2d 70 72 | 6f 74 65 69 6e 20 61 73 |Micro-pr|otein as|
|00005e90| 73 61 79 2c 4e 61 43 6c | 20 70 70 74 20 6f 66 20 |say,NaCl| ppt of |
|00005ea0| 52 4e 41 2c 4e 69 63 6b | 20 74 72 61 6e 73 6c 61 |RNA,Nick| transla|
|00005eb0| 74 69 6f 6e 2c 4e 6f 72 | 74 68 65 72 6e 73 2c 4f |tion,Nor|therns,O|
|00005ec0| 6c 64 20 48 79 62 72 69 | 64 69 7a 61 74 69 6f 6e |ld Hybri|dization|
|00005ed0| 2c 4f 6c 69 67 6f 28 64 | 54 29 2d 63 65 6c 6c 75 |,Oligo(d|T)-cellu|
|00005ee0| 6c 6f 73 65 2c 70 43 70 | 2d 6c 61 62 65 6c 69 6e |lose,pCp|-labelin|
|00005ef0| 67 20 52 4e 41 2c 50 6f | 6c 79 28 41 29 20 61 73 |g RNA,Po|ly(A) as|
|00005f00| 73 61 79 2c 50 6f 6c 79 | 28 55 29 20 73 65 70 68 |say,Poly|(U) seph|
|00005f10| 61 72 6f 73 65 2c 50 6f | 6c 79 28 55 29 20 73 75 |arose,Po|ly(U) su|
|00005f20| 63 72 6f 73 65 20 67 72 | 61 64 69 65 6e 74 73 2c |crose gr|adients,|
|00005f30| 50 72 69 6d 65 72 20 65 | 78 74 65 6e 73 69 6f 6e |Primer e|xtension|
|00005f40| 2c 52 62 2b 2b 20 63 6f | 6d 70 65 74 65 6e 74 20 |,Rb++ co|mpetent |
|00005f50| 45 2e 63 6f 6c 69 2c 52 | 4e 41 20 73 75 63 72 6f |E.coli,R|NA sucro|
|00005f60| 73 65 20 67 72 61 64 69 | 65 6e 74 73 2c 52 4e 41 |se gradi|ents,RNA|
|00005f70| 20 70 6f 6c 79 6d 65 72 | 61 73 65 20 61 73 73 61 | polymer|ase assa|
|00005f80| 79 2c 52 4e 61 73 65 20 | 50 20 52 4e 41 20 70 72 |y,RNase |P RNA pr|
|00005f90| 65 70 2c 53 65 71 75 65 | 6e 63 69 6e 67 20 67 65 |ep,Seque|ncing ge|
|00005fa0| 6c 73 2c 53 69 6e 67 6c | 65 2d 73 74 65 70 20 52 |ls,Singl|e-step R|
|00005fb0| 4e 41 20 69 73 6f 6c 61 | 74 69 6f 6e 2c 53 6f 75 |NA isola|tion,Sou|
|00005fc0| 74 68 65 72 6e 73 2c 53 | 70 6f 74 20 62 6c 6f 74 |therns,S|pot blot|
|00005fd0| 73 2c 53 70 69 6e 20 63 | 6f 6c 75 6d 6e 73 2c 53 |s,Spin c|olumns,S|
|00005fe0| 53 20 44 4e 41 20 70 72 | 65 70 20 66 72 6f 6d 20 |S DNA pr|ep from |
|00005ff0| 42 6c 75 65 73 63 72 69 | 70 74 2c 53 74 61 6e 64 |Bluescri|pt,Stand|
|00006000| 61 72 64 20 6c 69 67 61 | 74 69 6f 6e 2c 54 37 20 |ard liga|tion,T7 |
|00006010| 74 72 61 6e 73 63 72 69 | 70 74 69 6f 6e 2c 54 41 |transcri|ption,TA|
|00006020| 42 20 72 65 73 74 2e 20 | 62 75 66 66 65 72 2c 54 |B rest. |buffer,T|
|00006030| 72 61 6e 73 66 6f 72 6d | 61 74 69 6f 6e 2c 55 56 |ransform|ation,UV|
|00006040| 20 66 69 78 69 6e 67 20 | 66 69 6c 74 65 72 73 2c | fixing |filters,|
|00006050| 59 65 61 73 74 20 52 4e | 41 20 69 73 6f 6c 61 74 |Yeast RN|A isolat|
|00006060| 69 6f 6e 2c 41 64 76 61 | 6e 63 65 64 20 6d 69 6e |ion,Adva|nced min|
|00006070| 69 2d 70 72 65 70 2c 42 | 61 74 63 68 20 6f 6c 69 |i-prep,B|atch oli|
|00006080| 67 6f 28 64 54 29 2c 63 | 44 4e 41 20 73 79 6e 74 |go(dT),c|DNA synt|
|00006090| 68 65 73 69 73 2c 45 78 | 6f 20 49 49 49 20 73 65 |hesis,Ex|o III se|
|000060a0| 71 75 65 6e 63 69 6e 67 | 20 64 65 6c 65 74 69 6f |quencing| deletio|
|000060b0| 6e 73 2c 64 73 44 4e 41 | 20 73 65 71 75 65 6e 63 |ns,dsDNA| sequenc|
|000060c0| 69 6e 67 00 4d 26 4d 20 | 49 6e 74 72 6f 64 75 63 |ing.M&M |Introduc|
|000060d0| 74 69 6f 6e 00 6f 6e 20 | 6f 70 65 6e 43 61 72 64 |tion.on |openCard|
|000060e0| 0d 73 65 74 20 74 68 65 | 20 6c 6f 63 6b 54 65 78 |.set the| lockTex|
|000060f0| 74 20 6f 66 20 62 61 63 | 6b 67 72 6f 75 6e 64 20 |t of bac|kground |
|00006100| 66 69 65 6c 64 20 22 70 | 72 6f 74 6f 63 6f 6c 46 |field "p|rotocolF|
|00006110| 69 65 6c 64 22 20 74 6f | 20 74 72 75 65 0d 70 61 |ield" to| true.pa|
|00006120| 73 73 20 6f 70 65 6e 43 | 61 72 64 0d 65 6e 64 20 |ss openC|ard.end |
|00006130| 6f 70 65 6e 43 61 72 64 | 0d 0d 6f 6e 20 63 6c 6f |openCard|..on clo|
|00006140| 73 65 43 61 72 64 0d 73 | 65 74 20 74 68 65 20 6c |seCard.s|et the l|
|00006150| 6f 63 6b 54 65 78 74 20 | 6f 66 20 62 61 63 6b 67 |ockText |of backg|
|00006160| 72 6f 75 6e 64 20 66 69 | 65 6c 64 20 22 70 72 6f |round fi|eld "pro|
|00006170| 74 6f 63 6f 6c 46 69 65 | 6c 64 22 20 74 6f 20 66 |tocolFie|ld" to f|
|00006180| 61 6c 73 65 0d 65 6e 64 | 20 63 6c 6f 73 65 43 61 |alse.end| closeCa|
|00006190| 72 64 0d 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |rd......|........|
|000061a0| 00 00 16 60 43 41 52 44 | 00 00 4b 2c 00 00 00 00 |...`CARD|..K,....|
|000061b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000061c0| 00 00 4d 3b 00 00 0a 18 | 00 00 00 00 00 00 00 00 |..M;....|........|
|000061d0| 00 01 00 00 16 14 00 06 | 16 10 80 0a 00 00 00 0e |........|........|
|000061e0| 00 1e 00 01 53 45 51 55 | 45 4e 43 49 4e 47 20 44 |....SEQU|ENCING D|
|000061f0| 4f 55 42 4c 45 2d 53 54 | 52 41 4e 44 45 44 20 44 |OUBLE-ST|RANDED D|
|00006200| 4e 41 0d 0d 41 20 76 61 | 72 69 65 74 79 20 6f 66 |NA..A va|riety of|
|00006210| 20 73 65 71 75 65 6e 63 | 69 6e 67 20 70 72 6f 63 | sequenc|ing proc|
|00006220| 65 64 75 72 65 73 20 61 | 72 65 20 61 76 61 69 6c |edures a|re avail|
|00006230| 61 62 6c 65 20 2d 20 69 | 6e 20 6f 75 72 20 68 61 |able - i|n our ha|
|00006240| 6e 64 73 2c 20 74 68 65 | 20 62 65 73 74 20 6f 66 |nds, the| best of|
|00006250| 20 74 68 65 73 65 20 69 | 73 20 64 6f 75 62 6c 65 | these i|s double|
|00006260| 2d 73 74 72 61 6e 64 20 | 73 65 71 75 65 6e 63 69 |-strand |sequenci|
|00006270| 6e 67 20 75 73 69 6e 67 | 20 73 65 71 75 65 6e 61 |ng using| sequena|
|00006280| 73 65 20 61 6e 64 20 37 | 2d 64 65 61 7a 61 2d 64 |se and 7|-deaza-d|
|00006290| 47 54 50 2e 20 20 54 68 | 65 20 6d 65 74 68 6f 64 |GTP.  Th|e method|
|000062a0| 20 69 73 20 65 61 73 79 | 20 61 6e 64 20 72 65 6c | is easy| and rel|
|000062b0| 69 61 62 6c 65 2c 20 63 | 6f 6e 73 69 73 74 65 6e |iable, c|onsisten|
|000062c0| 74 6c 79 20 67 69 76 69 | 6e 67 20 33 30 30 20 6e |tly givi|ng 300 n|
|000062d0| 75 63 6c 65 6f 74 69 64 | 65 73 20 6f 66 20 63 6c |ucleotid|es of cl|
|000062e0| 65 61 72 2c 20 63 6c 65 | 61 6e 20 72 65 61 64 61 |ear, cle|an reada|
|000062f0| 62 6c 65 20 73 65 71 75 | 65 6e 63 65 2e 20 20 55 |ble sequ|ence.  U|
|00006300| 6e 6c 69 6b 65 20 6d 61 | 6e 79 20 64 73 44 4e 41 |nlike ma|ny dsDNA|
|00006310| 20 73 65 71 75 65 6e 63 | 69 6e 67 20 70 72 6f 63 | sequenc|ing proc|
|00006320| 65 64 75 72 65 73 2c 20 | 73 75 70 65 72 63 6f 69 |edures, |supercoi|
|00006330| 6c 65 64 20 44 4e 41 20 | 69 73 20 75 73 65 64 2e |led DNA |is used.|
|00006340| 20 20 49 6e 20 66 61 63 | 74 2c 20 6d 69 6e 69 70 |  In fac|t, minip|
|00006350| 72 65 70 20 44 4e 41 20 | 69 73 6f 6c 61 74 65 64 |rep DNA |isolated|
|00006360| 20 75 73 69 6e 67 20 4e | 61 4f 48 2d 53 44 53 20 | using N|aOH-SDS |
|00006370| 70 72 6f 63 65 64 75 72 | 65 73 20 77 6f 72 6b 73 |procedur|es works|
|00006380| 20 61 73 20 77 65 6c 6c | 20 61 73 20 43 73 43 6c | as well| as CsCl|
|00006390| 2d 70 75 72 69 66 69 65 | 64 20 44 4e 41 2e 20 20 |-purifie|d DNA.  |
|000063a0| 54 68 65 20 70 72 6f 63 | 65 64 75 72 65 20 69 73 |The proc|edure is|
|000063b0| 2c 20 68 6f 77 65 76 65 | 72 2c 20 71 75 69 74 65 |, howeve|r, quite|
|000063c0| 20 73 65 6e 73 69 74 69 | 76 65 20 74 6f 20 74 68 | sensiti|ve to th|
|000063d0| 65 20 68 6f 73 74 2d 73 | 74 72 61 69 6e 20 6f 66 |e host-s|train of|
|000063e0| 20 45 2e 63 6f 6c 69 20 | 2d 20 44 48 35 61 6c 70 | E.coli |- DH5alp|
|000063f0| 68 61 20 77 6f 72 6b 73 | 20 76 65 72 79 20 77 65 |ha works| very we|
+--------+-------------------------+-------------------------+--------+--------+